molecular cloning of clostridium perfringens type b vaccine strain beta toxin gene in e. coli

Authors

nariman shariatpanahi saveh, iran

reza pilehchian langroudi po. box 31375-1658, karaj, alborz, iran

abstract

clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. c. perfringens is subdivided to 5 groups (types a to e), based on its four major toxin (alpha, beta, epsilon and iota). c. perfringens type b beta toxin causes inflammation and bloody necrotic enteritis. type b related enterotoxaemia is a major problem of veterinary sciences. the aim of the present study was to molecular cloning and sequencing of c. perfringens type b vaccine strain beta toxin gene. genomic dna was extracted using phenol-chloroform method. beta toxin sequence was retrieve from genbank and using oligo software, appropriate primers were designed. using pfu dna polymerase, beta toxin gene was amplified and after purification it was ligated into pjet1.2/blunt cloning vector. the ligation product was transformed using e. coli/top10 competent cells and the recombinant pjetβ clones were chosen on lb-amp. pjetβ recombinant plasmid was extracted from recombinant bacterium host and was sequenced using universal primers. sequencing and blast and phylogenetic analysis of cpb showed over 99% identity to other previously deposited cpb in the genbank.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Molecular Cloning of Clostridium Perfringens type B Vaccine Strain Beta Toxin Gene in E. coli

Clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. C. perfringens is subdivided to 5 groups (types A to E), based on its four major toxin (alpha, beta, epsilon and iota). C. perfringens type B beta toxin causes inflammation and bloody necrotic enteritis. Type B related enterotoxaemia is a major problem of veterinary sciences...

full text

Molecular cloning of Clostridium septicum vaccine strain alpha toxin gene in E. coli

Clostridium septicum a Gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. C. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. The aim of the present study was to molecular cloning and sequencing of C. septicum vaccine strain alpha toxin gene. Genomic DNA was extracted using standard phenol and chlorofor...

full text

Cloning of Clostridium perfringens iota toxin gene in Escherichia coli

Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...

full text

Fusion of Clostridium perfringens type D and B epsilon and beta toxin genes and it’s cloning in E. coli

Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene. A good designed fusion gene construction could be cloned into a good and suitable host. Clostridium perfringens is an important pathogen of humans and livestock and produce...

full text

molecular cloning of clostridium septicum vaccine strain alpha toxin gene in e. coli

clostridium septicum a gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. c. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. the aim of the present study was to molecular cloning and sequencing of c. septicum vaccine strain alpha toxin gene. genomic dna was extracted using standard phenol and chlorofor...

full text

fusion of clostridium perfringens type d and b epsilon and beta toxin genes and it’s cloning in e. coli

designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. this construction should have all necessary components of a real gene. a good designed fusion gene construction could be cloned into a good and suitable host. clostridium perfringens is an important pathogen of humans and livestock and produce...

full text

My Resources

Save resource for easier access later


Journal title:
مجله بین المللی زیست و زیست پزشکی

جلد ۱، شماره ۱، صفحات ۲۹-۳۴

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023